Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTSEKQVKPIEIDDINIIENEKTKKAITAAALGNAIEWFDFGVYGYVAYVLGKVFFPDAS--PSVQMIAALATFSVPFIFRPLGGLFFGHLGDKYGRQKVLAITVIIMSISTFGIGLIPSY----------ETIGLWAPILLLIVKIVQGFSIGGEYSGAAIFVAEYSPDRKRGFMGSWLDFGSIAGFVLGAATVALITHAVGEARFAEWGWRIPFFLALPLGIIGLYLRNRLEETPVYQQHSEQQAQKS------------KPQKFSFKEIF---VKHKRSLLV--------C-IGLVISTNVTYYMLLTYLPSYFSHNLGYSEAHGALIIIAVMVGMLFVQPVIGYLSDKFGRRPFIFIGSFSLIFLSYPAFVLL-N-S-GVNYQIFIGLLILALSLNMSIGVMASTLPALFPTEIRYSALGIAFNFSVVIAGLTPTLTATLVETTHNLMVPAYYLMICGVVGIATAMYLKETANKPLIGGVPIAHNIEEAQELLEEFHETIEQKIENIDQKIQELQQKRQKLADKHPDIA 4GC0 Chain:A ((12-429)) -------------------------SITLVATLGGLLFGYDTAVISGTVESLNTVFVAPQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGSAWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIRGKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLYTVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFK-TLGASTDIALLQTIIVGVINLTFTVLAIMTVDKFGRKPLQIIGALGMAI-GMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMM-----------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4GC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1722 -190365 -110.55 -502.28 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -110.55 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.273

(partial model without unconserved sides chains): PDB file : Tito_4GC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GC0-query.scw PDB file : Tito_Scwrl_4GC0.pdb: