Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTFVDAARSFRTQFDLNFSEKIIVDFFAGGGGASTGLEMGLNR--PVYVAVNHNPKAISMHEANHPHAKHYIQDVFAVDPIDICDGHQVGWFHASPDCTHHSQAAGGQPRKKEIRDLSWVVLKFAGKV-KPDVISLENVKQILGWGPLIAKRDKATGRVITLDKININGKKVNRIAEPGERVPRHNQFLVPNPKKKGKTWKHFVRSLEQLGYEVEWQKNIIAADFGAPTKRERLFLIARCDGQPIVWPEKYFSKKP---KGNLKKWRSTVECVDFSDLGNSIFDRPQGPLADATLKRIAKGIQKYVIETKEPFFVNSSTPFIGRDFRTSFGHDIREPLATTTAGYGGHSSLISPILVPFITEFANASQQRNWSIDEPLSTICAQVKGGHHGLVTAKLSKDNYKGALRVAAFLINYYGNGDARSITEPMDTITTKDRLVLVTVWIKG-EPWAIVDICIRMLKPRELFRAQGVPDSYVIEYGSDGKPL-SKKDQVFMVGNSVSPYPMAAIARANNPFITQQIKGAA 4H0N Chain:B ((3-333)) --------------------SHKILELYSGIGGMHCAWKES-GLDGEIVAAVDINTVANSVYKHNFPETNLLNRNIQQLTPQVI-KKWNVDTILMSPPCQPFTRNGKYLDDNDPRTNSFLYLIGILDQLDNVDYILMENVKGFENST----------------------------------------------------VRNLFIDKLKECNFIYQEFL-LCPSTVGVPNSRLRYYCTARRNNLT--WPFKRRDEIITRLPKDFGVPHSLESIIEEDVD-EK-------FLVPEKMLRCAKVFD-ICY----------------------------KT--------------------------------SKRSC-----CF-TK----AYT------------------HYA-DGTG---SIFTDKPREVVQKCYAAAAQNEIGGEKFVELFKELKLRYFTPKEVLMIMCFPKSYNLP------TNISMKQCYRLLGNSVNVKVISELLKILFE----------

General information: TITO was launched using: RESULT: Template: 4H0N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1600 -7981 -4.99 -24.71 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : -4.99 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_4H0N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H0N-query.scw PDB file : Tito_Scwrl_4H0N.pdb: