Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQYSMRFWKNKYFLLIMLCLGVISPSVYAQSFDQNFQEWKAKQQMYDQKLNISKSSHSNSSKISQTKNFNDSTGQIHLNQANV----------NEFQQL---KGVG----EKKAQAIVEYRQ-------------------------KNGSFKNIDEIKNVKGIGPAIFEKNKSRLAL 3CI0 Chain:K ((65-187)) --------------------------------------------------------------GQIAVTLRDAQACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRLGREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCA

General information: TITO was launched using: RESULT: Template: 3CI0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain K - contact count / total energy / energy per contact / energy per residue : 188 5459 29.03 73.76 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain K : 0.60 3D Compatibility (PKB) : 29.03 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.590

(partial model without unconserved sides chains): PDB file : Tito_3CI0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CI0-query.scw PDB file : Tito_Scwrl_3CI0.pdb: