Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHWLEFMGNHPILFGTLGVLIVLFFIFEGQRNGRKISPQSLGILVKAKNALLIDLRDSKDFREGHISGSRNIPYSQIASHADELKASDRPLVFICNLGQV---AGSALQKVAHHDSYRLDGGISNWKAQGLPLVKSKPKA 3GK5 Chain:A ((101-198)) ---------------------------------YRSINAADLYENI--KAYTVLDVREPFELIFGSIANSINIPISELREKWKILE-RDKKYAVICAHGNRSAAAVEFLSQLGLN-IVDVEGGIQSWIEEGYPVV------

General information: TITO was launched using: RESULT: Template: 3GK5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 424 -49099 -115.80 -516.83 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -115.80 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_3GK5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GK5-query.scw PDB file : Tito_Scwrl_3GK5.pdb: