Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSAVVVSSENLDGQQQSSSTPASPAAEKVNLLGMSRAELEKFFEDIGEKKFRAGQVMKWIHQYFVTDFAEMTNISGKLRAKLEQICEIKAPEVVHRHYSKDGTRKWVFRVGEGSGSLVETVLIPAEDKTGSRKTLCISSQVGCALDCSFCSTGKQG--------FQRDLTPDEIIGQLWMANYSYMEEVPVAERERSVTNVVMMGMGEPLLNYDAVLSSMHIMLDDFAYGMSKRRVTLSTSGVVP--KIDQLAKDIDV-ALAISLHAPNDELRNELVPINKKYPLAQLIAACQRYIAKDGNESARKHVTIEYVMLEGVNDQPEHAQQLLKLLKNLPSKINLIPFNPFPHAPYGRSSRNRIISFQKTLSDAGFVCTIRQTRGDDIDAACGQLVGQVADRTRRAEQWQKKVAQRQEILRTQG 1TV8 Chain:B ((14-201)) -------------------------------------------------------------------------------------------------------------------------------------RDLRLSVTDRCNFRCDYCMPKEVFGDDFVFLPKNELLTFDEMARIAKVYAE------------LGVKKIRITG-GEPLMRRD-LDVLIAKLNQIDGI----EDIGLTTNGLLLKKHGQKLYD-AGLRRINVSLDAIDDTLFQSINNR--NIKATTILEQIDYATSI------GLNVKVNVVIQKGIN--DDQIIPMLEYFKDKHIEIRFIEFMDVGN-----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1TV8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 803 -8327 -10.37 -47.05 target 2D structure prediction score : 0.80 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -10.37 2D Compatibility (Sec. Struct. Predict.) : 0.80 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_1TV8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TV8-query.scw PDB file : Tito_Scwrl_1TV8.pdb: