Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDNATILQHVPVGKKVGIAFSGGLDTSAALLWMKQKGAEPYAYTANLGQPDEDDYDAIPKKAEQYGAVKARLIDCRLQLALEGI-AAIQCGAFHISTGGVPYFNTTPLGRAVTGTMLVTAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPNLKIYKPWLDQNFIDELGGRAEMSQFLIDNGFDYKMSKEKAYSTDSNMLGATHEAKDLEYLNAGIKIVDPIMGVAFWKEEVEIKPEEVTIRFEEGVPVAL----NG-QRFDNPVELILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQGRWFDSQALMLRETAQRWVAKAITGEVTLELRRGNDYTIMNTESPNLTYEAERLTMEKGDSMFTPMDRIGQLTMRNLDITDTRAKLGIYTDAGLLSIGQGSALPQLDNKKK 1VL2 Chain:A ((2-403)) -------------KEKVVLAYSGGLDTSVILKWLCEKGFDVIAYVANVGQKD--DFVAIKEKALKTGASKVYVEDLRREFVTDYIFTALLGNAMY----EGRYLLGTAIARPLIAKRQVEIAEKEGAQYVAHGATGKGNDQVRFELTYAALNPNLKVISPWKDPEFLAKFK--TDLINYAMEKGIPIKVSKKRPYSEDENLMHISHEAGKLEDPAHIPDEDVFTWTVSP--KDAPDEETLLEIHFENGIPVKVVNLKDGTEKTD-PLELFEYLNEVGAKNGVGRLDMVENRFIGIKSRGVYETPGATILWIAHRDLEGITMDKEVMHLRDMLAPKFAELIYNGFWFSPEMEFLLAAFRK-AQENVTGKVTVSIYKGNVMPV-ARYSPYSLYNP---------GGFDATDSKGFINIHALRLKVHQLV--------------------------

General information: TITO was launched using: RESULT: Template: 1VL2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2023 -10832 -5.35 -28.06 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -5.35 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.416

(partial model without unconserved sides chains): PDB file : Tito_1VL2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VL2-query.scw PDB file : Tito_Scwrl_1VL2.pdb: