Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLEFTKMHGLGNDFMVVD-LISQRAYLDTATIQRLADRHFGVGFDQLLIVEPPDVPEADFKYRIFNADGSEVEQCGNGVRCFARFVHE-RHLTNKTNITVQTKAGIVKPELGQNGWVRVNMGYPKFLPNEIP--FVADEPEALYTLELANDQ-NISIDVVNMGNPHAVTIV----PDVLTADV--AGIGPQVESHKRFPERINAGFMQVIDDKHVRLRVFERCVGETLACGTGACAAAVSGMRRGLLANSVEVELAGGKLQIEW-QEGDVVWMTGPTTHVYDGRLDLRYFQG 3EJX Chain:C ((26-307)) -VLHFVKYHGLGNDFILVDNRDSSEPKITQEQAAKLCDRNFGVGADGV-IFAMPGVNGTDYAMRIFNSDGSEPEMCGNGVRCFARFIAELENLQGKHSFTIHTGAGLIVPEIQDDGQVKVDMGTPILKAQDVPTKLSGNKGEAVVEAELVVDGVSWNVTCVSMGNPHCITFGKKGGPNLKVDDLNLPEIGPKFEHHEMFPARTNTEFVEVLSRSHLKMRVWERGAGATLACGTGACALVVAAVLEGRADRKCTVDLPGGPLEIEWKQEDNHIYMTGPAEAVFYG---------

General information: TITO was launched using: RESULT: Template: 3EJX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1667 -17066 -10.24 -63.21 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.81 3D Compatibility (PKB) : -10.24 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_3EJX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EJX-query.scw PDB file : Tito_Scwrl_3EJX.pdb: