Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MTDLNKKREVNLSFE-----QDDGA---VWVFDGDSHQGTEISHLMMMHSDEYNEDELRVICNHAAF------------EIDRLRAEL------EKSKAQAVPEAQQKLTDTYFWEGSDYVVDCP------FEYDIELDKGEVLELQKWQRTESTKVYFANIYKDEDNFEIL-QFASKA-------EAENAVAENLKFLEASESGAEG 3J6N Chain:K ((25-233)) PEQRIEKAKGETAYLPCKFTLSPEDQGPLDIEWLISPSDNQIVD-QVIILYSGDKIYDNYYPDLKGRVHFTSNDVKSGDASINVTNLQLSDIGTYQCKVKKAPGVANKKFLLTVLVKPSGTRCFVDGSEEIGNDFKLKCEPKEGSLPLQFEWQKLSDSQTMPTPWLAEMTSPVISVKNASSEYSGTYSCTVQNRVGSDQCMLRLDVVPPS-

General information: TITO was launched using: RESULT: Template: 3J6N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain K - contact count / total energy / energy per contact / energy per residue : 649 62784 96.74 378.21 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain K : 0.66 3D Compatibility (PKB) : 96.74 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.234

(partial model without unconserved sides chains): PDB file : Tito_3J6N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3J6N-query.scw PDB file : Tito_Scwrl_3J6N.pdb: