Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKRTPISFEFFPPKTDASAEKLRIVHQELQHLNPEFFSITYGAGGSTRERTLAAIEDFNGK-GTPVAPHLSCIGDDKTRIAELLDLYKAQGIDRIVALRGDLPSGQVGLGELPYAQDLVRFIREHSGDHFHIEVAAYPEMHPQAESLDSDIQRFIEKVQAGANAGITQFFFNPDSYFYFIERLEKAGINIPVAPGIMPITNASNLIRFADGTGAEIPRWIRKQLQAYGDDSESIKAFGHEVVVKLCERLIAGGAPSLHFYSMNQVEPTRQLVVDLGLN 1ZRQ Chain:B ((25-292)) ------VSFEFFPPRTSEMEQTLWNSIDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTIARDYWNNGIRHIVALRGDLPP------EM-YASDLVTLLKEVAD--FDISVAAYPEVHPEAKSAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYTLNRAEMSYAICHTLGV-

General information: TITO was launched using: RESULT: Template: 1ZRQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1313 -41774 -31.82 -159.44 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -31.82 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.657

(partial model without unconserved sides chains): PDB file : Tito_1ZRQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZRQ-query.scw PDB file : Tito_Scwrl_1ZRQ.pdb: