Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAVNASFTDYKVADISLADYGRKEIKLAEAEMPALIGLRKRHAASKPLAGAKILGCIHMTIQTAVLIETLVELGAEVRWTSCNIFSTQDHAAAAIATRGIPVFAWKGETEEEYVWCLEQQINVNGQPWDANMILDDGGDLTALVHEKYPALLERIHGITEETTTGVQRLIEMWKDGTLKVPAINVNDSVTKSKNDNKYGCRHSLNDAIKRATDMLLSGRRALVIGYGDVGKGSAQSLRQEGMVVRVTEVDPICAMQACMDGYEVVSPYKNGVQTGKKEDINHDLLGNTDLVVTTTGNYHVCDAAMLDSLKAGAVVCNIGHFDTEIDTAYL--RGYKWVEVKPQVHQVYRSEDENNYLILLSEGRLVNLGNATGHPSRVMDGSFANQVLGQIHLFQEKFADLPASEKAAKIRVEVLPKKLDEEVAAAMVAGFGGVLTQLTQEQADYLGVAVEGPFKSDAYKY 5W4B Chain:B ((4-429)) ----------YKVADIGLAAWGRKALDIAENEMPGLMRMRERYSASKPLKGARIAGCLHMTVETAVLIETLVTLGAEVQWSSCNIFSTQDHAAAAIAKAGIPVYAWKGETDEEYLWCIEQTLYFKDGP--LNMILDDGGDLTNLIHTKYPQLLPGIRGISEETTTGVHNLYKMMANGILKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQALRGFGARVIITEIDPINALQAAMEGYEVTT-MDEACQEG-------------NIFVTTTGCIDIILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDR-YRLKN-GRRIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHP--------DKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHYRY

General information: TITO was launched using: RESULT: Template: 5W4B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2468 -182598 -73.99 -430.65 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.87 3D Compatibility (PKB) : -73.99 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_5W4B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5W4B-query.scw PDB file : Tito_Scwrl_5W4B.pdb: