Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------MNDEYKNDEDKM-LFEEIENRC-RLNFELRGK--------------MSLIQQKKYLANKSEFTLGHVEKLISDWISSRS---------EFTKIKQPIKF-DMKKLLLNKSEIGNRDQYIRAKGQEIIDSLGEM-----RSYNYLYVTHRADGMVITVGK-SSSN-DIFLDGDLFYQLNTNHLSGTENIILRT-EYGNEIFAKYDEIL-KN-----YLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ 3L0I Chain:A ((23-347)) SFSNMVSAKKFYNKAIFTAPKAEVSVKTHIMRPIDFMLMGLREEFNLYSEDHLSAPGTIRLLREKNLLPEEQIARIESVYNQAMSKRFELHAEHEHDEMPYSDAKAMLDEVAKIRELGVQRVTRIENLENAKKLWDNANSMLEKGNISGYLKAANELHKFMKEKNLKEDDLRPELSDKTISPKGYAILQSLWGAASDYSRAAATLTESTVEPGLVSAVNKMSAFFMDCKLSPNERATPDPDFKVGKSKILVGIMQFIKDVADPTSKIWMHNTKALMNHKIAAIQKLERSNNVN-DETLESVLSSKGENLSEYLSYKY

General information: TITO was launched using: RESULT: Template: 3L0I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 693 68696 99.13 319.52 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 99.13 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.182

(partial model without unconserved sides chains): PDB file : Tito_3L0I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L0I-query.scw PDB file : Tito_Scwrl_3L0I.pdb: