Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPDKEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWG----EHRLEKNCGSHHIHGGTKGWSF---QFWEVEVFKTPSSIGVVFY--LKDEFSTYPGPITATITYRLTKNELEMTTSA-SSLVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPIDFRKSPTLQEILACYP-DGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFSTTGFEADF-SVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSAQ 1SNZ Chain:A ((16-341)) ------------GGTVEKFQLQSDLLRVDIISWGCTITALEVKDRQGRASDVVLGFAELEGYLQKQPYFGAVIGRVANRIAKGTFKVDGKEYHLAINKEPNSLHGG---VRGFDKVLWTPRVLS----NGVQFSRISPDGEEGYPGELKVWVTYTLDGGELIVNYRAQA-SQATPVNLTNHSYFNLAGQASPNINDHEVTIEADTYLPVDETLIPTGEVAPVQGTAFDLRKPVELGKHLQDFHLNGFDHNFCLKGSKEKHFCARVHHAASGRVLEVYTTQPGVQFYTGNFLDGTLKGKNGAVYPKHSGFCLETQNWPDAVNQPRFPPVLLRPGEEYDHTTWFKFSV-

General information: TITO was launched using: RESULT: Template: 1SNZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1884 -15563 -8.26 -49.56 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -8.26 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_1SNZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SNZ-query.scw PDB file : Tito_Scwrl_1SNZ.pdb: