Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQTNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIERVKETYGRH 5T4O Chain:I ((54-136)) ------------------DLAKASATDQLKKAKAEAQVIIEQANKR----RSQILDEAKAEAEQERTKIVAQAQAEIEAERKRAREELRKQVAILAVAGAEKIIE-----

General information: TITO was launched using: RESULT: Template: 5T4O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 11 -102 -9.23 -1.22 target 2D structure prediction score : 0.86 Monomeric hydrophicity matching model chain I : 0.67 3D Compatibility (PKB) : -9.23 2D Compatibility (Sec. Struct. Predict.) : 0.86 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.736

(partial model without unconserved sides chains): PDB file : Tito_5T4O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T4O-query.scw PDB file : Tito_Scwrl_5T4O.pdb: