Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQEMMEIVQQKQLAPKIFEMTLKGDL-VKEMQQPGQFLHILVPRAD-----------------------------LLLRRPISLNHIDHETNTCRIIYRV-----------EGEGTRVFSTLSAGDHLDVMGPLGNGFDLSNLHTGDEVFIIGGGIGVPPLYELSKQLVEK---GIKPTHFLGFATHEVVYYEKEFNRL-G---E--TRIA-TD----DGTYGMHGNVGNLLLG-TK-----TEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRMACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII 4P6V Chain:F ((132-394)) --KWECTVISNDNKATFIKELKLAIPDGESVPFRAGGYIQIEAPAHHVKYADFDVPEKYRGDWDKFNLFRYESKVDEPIIRAYSMANYPEEFGIIMLNVRIATPPPNNPNVPPGQMSSYIWSLKAGDKCTISGPFGEF-FAKDT--DAEMVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSYWYGARSKREMFYVEDFDGLAAENDNFVWHCALSDPQPEDNWTGYTGFIHNVLYENYLKDHEAPEDCEYYMCGPPMMNAAVINMLKNL---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4P6V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 995 15905 15.98 78.74 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain F : 0.66 3D Compatibility (PKB) : 15.98 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.396

(partial model without unconserved sides chains): PDB file : Tito_4P6V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P6V-query.scw PDB file : Tito_Scwrl_4P6V.pdb: