Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRKDEHVSLAKAFH---DKQKNEFDFVRIIHNPLPQIAVSDVDLSTQAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADTYTIMRQEYPHGKIIANIGAGTS-----VERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT-NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENARRSK-----RELSY-LADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGTSGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCEARGIDLVKYGRRTEKSVGQK 3B05 Chain:D ((5-364)) VNRKVEHVEIAAFENVDGLSSSTFLNDVILVHQGFPGISFSEINTKTKFFRKEISVPVMVTGMTGGRNELGRINKIIAEVAEKFGIPMGVGSQRVAIEKAEARESFAIVRKVAPTIPIIANLGMPQLVKGYGLKEFQDAIQMIEADAIAVHLNPAQEVFQPEGEPEYQIYALEKLRDISKELSVPIIVKESGNGISMETAKLLYSYGIKNFDTSGQGGTNWIAIEMIRDIRRGNWKAESAKNFLDWGVPTAASIMEVRYSVPDSFLVGSGGIRSGLDAAKAIALGADIAGMALPVLKSAI-EGKESLEQFFRKIIFELKAAMMLTGSKDVDALKKTSIVILGKLKEWAEYRGINLSIYEKV--------

General information: TITO was launched using: RESULT: Template: 3B05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2150 2821 1.31 8.18 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 0.77 3D Compatibility (PKB) : 1.31 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.486

(partial model without unconserved sides chains): PDB file : Tito_3B05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3B05-query.scw PDB file : Tito_Scwrl_3B05.pdb: