Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQEDVKKIREH-A--SFDIDRILEIEKLTRHDVVAFTRAVSETL---G-EERKWVHYGLTSTDVVDTAYGYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSEMKRNIERFEHAAKGVEAGKISGAVGTFANI---PPFIEEYVCKQLGIRPQEISTQVLPRDLHAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSENMAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNLTVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPLLEEDPTITSVLSKEEIDDAFDYHHHLK----NVDVIFKRVGLE 1RE5 Chain:D ((6-447)) FDAYFTAPAMREIFSDRGRLQGMLDFEAALARAEASAGLVPHSAVAAIEAACQAERYDTGALANAIATAGNSAIPLVKALGKVIATGVPEAERYVHLGATSQDAMDTGLVLQLRDALDLIEADLGKLADTLSQQALKHADTPLVGRTWLQHATPVTLGMKLAGVLGALTRHRQRLQELRPRLLVLQFGGASGSLAALGSKAMPVAEALAEQLKLTLPEQ-PWHTQRDRLVEFASVLGLVAGSLGKFGRDISLLMQTEAGEVFEPSAPGKGGSSTMPHKRNPVGAAVLIGAATRVPGLLSTLFAAMPQEHERSLGLWHAEWETLPDICCLVSGALRQAQVIAEGMEVDAARMRRNLDLTQGLVLAEAVSIVLA-QRLGRDRAHHLLEQCCQRAVAEQRHLRAVLGDEPQVSAELSGEELDRLLDPAHYLGQARVWVARAVSEHQR-

General information: TITO was launched using: RESULT: Template: 1RE5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2050 -7382 -3.60 -17.25 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 0.76 3D Compatibility (PKB) : -3.60 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_1RE5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RE5-query.scw PDB file : Tito_Scwrl_1RE5.pdb: