Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPKEKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSIAWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPNRKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHP---HLEQAKYQGAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQK-TIEQQRTSLFFSDVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPE--------NWEGVQGNLITGGI-------KNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDKVEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGYIYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL 4ZE4 Chain:B ((13-485)) LKPFPPEFLWGAASAAYQVEGAWNEDGKGLSVWDVFA------------------KQPGRTFKGTNGDV------AVDHYHRYQEDVALMAEMGLKAYRFSVSWSRVFPDGNGAV-NEKGLDFYDRLIEELRNHGIEPIVTLYHWDVPQALMDAYGAWESRRIIDDFDRYAVTLFQRFGDRVKYWVTLNEQNI-----FISFGYRLGLHPPGVKDMKRMYEANHIANLANAKVIQSFRHYVPDGKIGPSFAYSPMYPYDSRPENVLAFENAEEFQNHWWMDVYAWGMYPQAAWNYLESQGLEPTVAPGDWELLQAAKPDFMGVNYYQTTTVEHNPPDGVGEGVMNTTGKKGTSTSSGIPGLFKTVRNPHVDTTNWDWAIDPVGLRIGLRRIANRYQLPILITENGLGEFDTLEPGDIVNDDYRIDYLRRHVQEIQRAITDGVDVLGYCAWSFTDLLSW-LNGYQKRYGFVYVNRDDESEKDLRRIKKKSFYWYQRVIETNGAEL

General information: TITO was launched using: RESULT: Template: 4ZE4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2638 -64119 -24.31 -141.23 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -24.31 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_4ZE4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZE4-query.scw PDB file : Tito_Scwrl_4ZE4.pdb: