Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSW------NEWYMHTKYGNELNQATD----TVINGATGILMVEKMAAWILLAF-------AAFACFYI--GEEFQNGTIR-NTLSLGRNRMTYYVSKLLVTLLITTLGVVLITGLAM-------IAYTLAFGFGEVEGIKNYGNYVLKVFPVLLLLILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFA-----KIKGLEFLTEWFT---ETWLMY---TDFAQQATYSQAPKMILV----------------------SLVTIVVSSALGMFIFQKSDIK--- 5EZN Chain:A ((1-331)) --HVFIRTELSFIKNNVPCIRDMFFIYKRELYNICLDDDETHIYVQKKVKDSWITLNDLFKETDLTGRPHIFAYVDVEEIIILLCEDEEKKDMTCHRFYSNDGKEYQNSEITISDYILKDKLLSSYVS---LPLKIENREYFLICGVSPYKFKKDDILCMASHDKGETWGTKIVIKYDNYKLGVQYFFLRPYISKNDLSFHFYVGDNINNVKNVNFIECTHEKDLEFVCSNRDFLKDNKVLQDVSTLNDEYIVSYGNDNNFAECYIFFNNENSILIKPEKYGQTTAGCYGGTFVKIDEQRTLFIYSSSQGIYNIHTIYYANYE

General information: TITO was launched using: RESULT: Template: 5EZN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1229 -126823 -103.19 -497.35 target 2D structure prediction score : 0.16 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -103.19 2D Compatibility (Sec. Struct. Predict.) : 0.16 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.060

(partial model without unconserved sides chains): PDB file : Tito_5EZN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EZN-query.scw PDB file : Tito_Scwrl_5EZN.pdb: