Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNNTTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLILAAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQ-KG-ATFIGTNPDKNIPTERGLLPGAGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRNNIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF 1YV9 Chain:B ((3-255)) LDYQGYLIDLDGTIYLGKEPIPAGKRFVERLQEKDLPFLFVTNNTTKSPETVAQRLANEFDIHVPASLVYTATLATIDYMKEANRGKKVFVIGEAGLIDLILEAGFEWDETNPDYVVVGLDT--ELSYEKVVLATLAIQKGALFIGTNPDKNIPTERGLLPGAGSVVTFVETATQTKPVYIGKPKAIIMERAIAHLGVEKEQVIMVGDNYETDIQSGIQNGIDSLLVTSGFTPKSAVPTLPTPPTYVVDSLDEWT-

General information: TITO was launched using: RESULT: Template: 1YV9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1416 -57079 -40.31 -227.41 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.90 3D Compatibility (PKB) : -40.31 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.641

(partial model without unconserved sides chains): PDB file : Tito_1YV9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YV9-query.scw PDB file : Tito_Scwrl_1YV9.pdb: