Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNEYGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNIL 3AON Chain:B ((1-101)) MTYKIGVVGDKDSVSPFRLFGFDVQHGTTKTEIRKTIDEMAKNEYGVIYITEQCANLVPETIERYKGQLTPAIILIPSHQGTLGIGLEEIQNSVEKAVGQN--

General information: TITO was launched using: RESULT: Template: 3AON.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 411 -57302 -139.42 -567.35 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.92 3D Compatibility (PKB) : -139.42 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.603

(partial model without unconserved sides chains): PDB file : Tito_3AON.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3AON-query.scw PDB file : Tito_Scwrl_3AON.pdb: