Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVAQAIRNIISPKSVIAQYQKIDFSNKGTIPIRHLNIGFD----GKEIDIEFYVNNQFATLFFATLSVFLTYGEDLVIETARHHREFIQD---PILKQRVTSLIGQEAIHSKLHNEFNDAIKELEYPVDLYRFLGENFFKYVFL-KFPQPLKLSLMAGIEHFTAVLA-----EYMMKHEKNFYYTDDAKSRALWMWH-MLEESEHKDIAYDVYQILNG------NYPLRATGFLMAFITILGLIPTATLLVPVLRKPKELLTRKFWKDARRGV--NLIFSPKDGVFGSTIGQIFDYLRPNFHPNDHDTTQYLEYYKNKLLNEGGAIAPYFVKEFTPPVRAVS 5JYG Chain:A ((14-341)) ----LGIDLGTSHTAVMSSRGKKFLLKSVVGYPKDVIGLKLLGRPYVVGDEAFEMRSYLDIRYPLQDGVLSEISDRDIEVARHLLTHVVKSAEPGPNDEICAVIGVPARASAANKALLLKMAQEVVHTALVVS--EPFMVGYGLDKLINTIIVDIGAGTTDICALKGTVPGPEDQVTLTKAGNYVDERLQNAILERHPELQMNVNVACAVKEQFSFVGTPTEVASFEFRAAGKPVRADVTEPVKIACEALMPDIIESIETLLRSFQPEYQATVLQNIVFAGG----GSRIRGLAAYVKEKLRPFGDANVTCVK----DPTFDGCRGALRLAEELPPQYWRQL

General information: TITO was launched using: RESULT: Template: 5JYG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1439 3540 2.46 11.57 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 2.46 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.103

(partial model without unconserved sides chains): PDB file : Tito_5JYG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JYG-query.scw PDB file : Tito_Scwrl_5JYG.pdb: