Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKSFAINMAPTNHGGMIPATQSRTSQEGNLFVRAGDGHMCPKCKCWSVVIKSHDHVIMDGKPVAYVGDKLSCGATIMPQQFHVVGDSGSPYSSAAPVQNSLVEDKSNEIHKVQFKLVDVDTDQPLSAMLYEIHSKESGKLLVQGYTDKNGMTAIYESEHTPESVQLITIDLSKPLDPL 1ZPS Chain:A ((8-131)) VNILLNFRHNINGEDLIIAVAQDHETGE----------------------------VLMVAYM-----NREALRRTLETGTAHYWSTSRGKLWLKGE--------SSGHVQRVKDVLVDCDGD----AVVLKVEQ--------EGGACHTGYRSCFYRSIDGDELKVRE-DAVKVFDP-

General information: TITO was launched using: RESULT: Template: 1ZPS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 567 -28741 -50.69 -231.78 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -50.69 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.154

(partial model without unconserved sides chains): PDB file : Tito_1ZPS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZPS-query.scw PDB file : Tito_Scwrl_1ZPS.pdb: