Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKISLLGSGRVAFHLAKALLAQGHHIAQVYARDFEKTKKFAEQI------QAKACQSLQEFQPTDLIILAVSDS-AI-TELATQVHELFPKMLMVHTSGSTDIQVISQVH---EKAGVFYPLQTFSLERDVDWKATPLFVEA--TNKDDLVILSDLANSLSKRVYQYTSKQRLTLHLAAVFACNFSNYCFDMAKQVVDAEQVDFGLLYPLILETAKKATENDPKQMQTGPAMRGDQNILVMHQSLLAQANRDDLKQVYQLLSDGIVKRHHSS 1ZEJ Chain:A ((13-236)) MKVFVIGAGLMGRGIAIAIA-SKHEVV-LQDVSEKALEAAREQIPEELLSKIEFTTTLEKVKDCDIVMEAVFEDLNTKVEVLREVERL-TNAPLCSNTSVISVDDIAERLDSPSRFLGVHWMNPPHV------MPLVEIVISRFTDSKTVAFVEGFLRELGKEVVVCKGQ--S-------LVNRFNAAVLSEASRMI-EEGVRAEDVDRVWKHHLGL------LYTLFGPLGNLDYIGLDVAYYASLYL-----------------------

General information: TITO was launched using: RESULT: Template: 1ZEJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 984 -49952 -50.76 -236.74 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -50.76 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.397

(partial model without unconserved sides chains): PDB file : Tito_1ZEJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZEJ-query.scw PDB file : Tito_Scwrl_1ZEJ.pdb: