Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRHYTGIDQLINSFDQALRSLVPGATAAQRQNPAETVEAKLGVEDARHVAGLMRVNHSGEVCAQALYHGQALTAKLP---NVRREMQQAAIEEQDHLAWCEDRLKELNSHTSLLNPIWYGLSYGMGALAGIAGDKYSLGFVAETERQVSLHLQDHLNQLPA-QDERSRKILEQMNEDELHHRHTALEAGGVELPYAVKITMTAISKLMTKTSYYL 1JI5 Chain:A ((3-137)) -------------------------------------------------IEVLNKQVADWSVLFTKLHNFHWYVKGPQFFTLHEKFEELYTESATHIDEIAERILAIGGKPVATMKEYLEISSIQEAAYG-ETAEGMVEAIMKDYEMMLVELKKGMEIAQNSDDEMTSDLLLGIYTELEKHAWML------------------------------

General information: TITO was launched using: RESULT: Template: 1JI5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 508 -1788 -3.52 -13.65 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -3.52 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.407

(partial model without unconserved sides chains): PDB file : Tito_1JI5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JI5-query.scw PDB file : Tito_Scwrl_1JI5.pdb: