Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MMKLLSFLKNKALGSSIDYKILPRKVKFDWKDTPIDWIPNQPFASYFINEINNILPAGEFWFCRLYNKVLPRITDEKLKQDVQAFIRQEAMHANAHTSANKEYLSARNIDIQRNLDIMNYLFTTALADKPFDKEVPQFLQEQWDLFRLGVIATVEHMTCVLGKYALYNKRWEELGADPEMVDLVKWHGSEEIEHRTVAFDLYRHLGGGYIPRYYLSLAVIVLVLGLWVDGAAHIMKQDPRFADAAKSRFFAAWVALEWYKISRKDNQVLPNPIWLIAQQIDYLMPWYDPVKEGSTEDAVSYLSQSPAAKRAELQAA---- 1UDD Chain:A ((1-215)) MRVMITDKLRRDS---------------------------EQIWKKIFEHPFVVQLYSGTL-----------------PLEKFKFYVLQDFNYLVGLTRALAVISSK--------------------AEYPLMAELIELARDE-----------------VTVEVENYVKLLKELDLTLEDAIKTE----PTLVNSAYMDFMLATAYKG-------NIIEGLTALLPCFWSYAEIAEYHKDKLRDNPIKIYREWGKVYLS--------------NEYLNLVGRLRKIIDSSGHSGYDRLRRIFITGSKFELAFWEMAWRGG

General information: TITO was launched using: RESULT: Template: 1UDD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 854 -17631 -20.64 -83.95 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -20.64 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_1UDD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UDD-query.scw PDB file : Tito_Scwrl_1UDD.pdb: