Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------MVSFLFIGL--VVTILL----TPGPTNTLLASAGIQAGVKHSLKLIPAEVIGYLIAITSWGVLLE-----SVSHFIPWLPAILKLMSAGFILYLAFKLWMT-SSEDIKLDRPL-ITPKALFLATLL------NPKALLFASAIFPHAAWEIPHL-----------YFIHIVVFLSLITPIAFLWIMFGTVLISNKVTWLNQRNLQRIAAFILTFFAMPIGYSAISSF 5EDF Chain:A ((2-225)) EQTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL-----IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPEYEAYEDKRHIPENPYFHEFYYIKKGENPAIITHRNNRINQTEEDSYSTSVGSCINGFTVQYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNN--------------

General information: TITO was launched using: RESULT: Template: 5EDF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 679 -38521 -56.73 -216.41 target 2D structure prediction score : 0.23 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -56.73 2D Compatibility (Sec. Struct. Predict.) : 0.23 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.019

(partial model without unconserved sides chains): PDB file : Tito_5EDF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EDF-query.scw PDB file : Tito_Scwrl_5EDF.pdb: