Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDLFLNRKSFVVKSLAITVTALMMSGANAATSDKEEIRKLRQEVEALKALVQEQRQVQQQQQQVQQQQQVQLAEVKAQPQPAAAPASPLASLKSKTGADVNLYGFVRGDANYIIEGADNDFADVSKSNGKTQDKLRATAKTTRLGLDFTAPV-SGAKVGGKIELDFASSDNDKSENVRIRHAYLTYN----NWLFGQTTSNFLS--SHAPE--MIDFST--NI------------GGGTTRIPQVRYNYKLAPATQLFVSAEEGNSSATGDSVKYRLPVLTAKVTQGYADGNGNASARAFVENYKSGAGDDKTGWGIAAGTDFKV-SDPLKLFADASYVVGNSNYLYGSNSAYTLVNDNIEQNEFVAVQVGGTYKILPNLRSTLAYGAQFADDGTDYAKNNVTANEKVQQAWINFIYTPVKPIDLGVEYVNGKRDTFEGKSYKDNRVGLMAKYSF 3PRN Chain:A ((1-289)) ---------------------------------------------------------------------------------------------------MISLNGYGRFGLQYVEDRGVG------------LEDTII-SSRLRINIVGTTETDQGVTFGAKLRMQWDDGDA--FAGTAGNAAQFWTSYNGVTVSVGNVDTAFDSVALTYDSEMGYEWSSFGDAQSSFFAYNSKYDASGALDNYNGIAVTYSISGVNLYLSYVDPDQTVD---SSLVTEEFGIAADWSN--DM--ISLAAAYTTDAGG-IVDNDIAF--VGAAYKFND-AGTVGLNWYDNGLS--------------------TAGDQVTLYGNYAFG-ATTVRAYVSDIDRA-------------GADTAYGIGADYQFAEGVKVSGSVQSGF-AN------E-TVADVGVRFDF

General information: TITO was launched using: RESULT: Template: 3PRN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1272 29609 23.28 111.73 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 23.28 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.109

(partial model without unconserved sides chains): PDB file : Tito_3PRN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PRN-query.scw PDB file : Tito_Scwrl_3PRN.pdb: