Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVHFLHTSDWHLGQFFYN----HSRHYEHQQFLSWLLTQIQEKQPHALLIAGDIFDV-INPGSQAQKQLYQFLADAHRIAPHMQTLMIAGNHDSGYRIEQVEPLL-EKYNAKTVGVVRWNEDKTLDLDRLLLPIYNQNQDIVAWCLALPFLRSAEITGFNEHTTNSKNAIAYLHQQLIAEAKRRKTPDQALILMSH-AHMQGGETSDSERPIIIGNEEALSTTLFEDAVDYVALGHLHKPQKVG-QPHIRYSGSPIPLSFSEINYKHQVVEVKIDPSQDIDSRLQFEAVEIPRCIQLHRIR---GELNEVLQQLKALPHGVIENIDHREYVDIEYYSLTP-PQPNLRQQFEAALPPDRYRLVRISRQYVNKDTT---NSNTTQHIALEPPTPEKLFQNIWEKQGYSADDAVLKDFLSLVQEAQKHLENDASH 3THO Chain:B ((1-377)) --MKILHTSDWHLGVTSWTSSRPVDRREELKKALDKVVEEAEKREVDLILLTGDLLHSRNNPSVVALHDLLDYLKRMM-R--TAPVVVLPGNQDWKGLKLFGNFVTSISSDITFVMSFEPVD----------VEAK--RG-QKVRILPFPYPDES----KNEGD--FRFFLESRLNKL-YEE--ALKKEDFAIFMGHFTVEGLAG-----------REIIINRALIPSVVDYAALGHIHSFREIQKQPLTIYPGSLIRIDFGEEADEKGAVFVELKRGEP----PRYERIDASP-LPLKTLYYKKIDTSALKS-IRDSC------RNFPGYVRVVYEEDSGILPDLMG-EIDNLV--------KIERKSRREIEEVLRESPEEFKEELDKLDYFELFKEYLKKRE-ENHEKLLKILDELLDEVKKS-------

General information: TITO was launched using: RESULT: Template: 3THO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1696 54407 32.08 155.01 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : 32.08 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.380

(partial model without unconserved sides chains): PDB file : Tito_3THO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3THO-query.scw PDB file : Tito_Scwrl_3THO.pdb: