Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTLSMNYLQDARQHVLQQIRTACELAQREPETVQLLAVSKTHPSERLREM-YAAGQRAFGENYLQEALDKIDALQDLDIEWHFIGHVQRNKTKHLAEK----FDWVHGVDRLIIAERLSNQRGDDQAALNICLQVNIDGQESKDGCAPEDV---AELVAQMSQLPKIKLRGLMVIPAPDNTG------------AFADAKKLFDAVKVQHAHPEDWDTLSMGMSSDLEAAIA-AGSTMVRVGTALFGARDYSQKG 3WQE Chain:B ((25-256)) ---IDLDRMQRNIARMQQRMD---------AQGVRLRPHVKTSKSVPVAAAQRAAGASGITVSTLKEAEQFFAA-GT-T-DILYAVSMAPHRLPQALQLRRRGCDLKLIVDSVAAAQAIAAFGREQGEAFEVWIEIDTDG--HRSGVGADDTPLLLAIGRTL-HDGGMRLGGVLTHAGSSYELDTPEALQALAERERAGCVQAAEALRAAG--L-PCPVVSVGSTPTALAASRLDGVTEVRAGVYVFFDLVMR---

General information: TITO was launched using: RESULT: Template: 3WQE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1064 10038 9.43 47.57 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : 9.43 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_3WQE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WQE-query.scw PDB file : Tito_Scwrl_3WQE.pdb: