Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSANTFPAQIEPFKWAEQGFTWSGQLPLSRFARIAREAVGSIDNQLINIDCKLSMDAYHRIVWLDGHMETKVPMECQRCLEPVEMPIVSDFHIALVDDESLIERLDEDADFIVLGESESSTKGDFDTPASINLLALLEDELLLLMPLSPKHDACEHKHQPTIQNVVEEKRDNPFDVLAGLKGKLNS 1JYO Chain:E ((36-137)) -----DKAYVAPEKFSSKVLTWLGKMPLFKNTEVVQKHTENIRVQDQKILQTFLHALTEK--YGETAVNDALLMSRINMNKPLTQRLAVQITECVKAADEGFINLIKSK-----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1JYO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 145 -8772 -60.49 -86.00 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain E : 0.60 3D Compatibility (PKB) : -60.49 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.254

(partial model without unconserved sides chains): PDB file : Tito_1JYO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JYO-query.scw PDB file : Tito_Scwrl_1JYO.pdb: