Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNTYIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITAFPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQILYQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGITRSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY 5EY2 Chain:C ((18-264)) -MELLAKTRKLNALLQSA----AGKP--FREMSDTMCEVIEANVFVVSRRGKLLGYAIHQQIENERMK--LAERQFPEEY-QSLFNITETS-SNLD---------------GQGLTTIVPIVGG----GTLVLARLGQEFLDDDLILAEYSSTVVGMEILREKAEEIEEEARSKAVVQMAISSLSYSELEAIEHIFEELNGTEGLLVASKIADRVGITRSVIVNALRKLESAGVIESRSLGMKGTYIKVLND-----------

General information: TITO was launched using: RESULT: Template: 5EY2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 859 9798 11.41 44.33 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.83 3D Compatibility (PKB) : 11.41 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.173

(partial model without unconserved sides chains): PDB file : Tito_5EY2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EY2-query.scw PDB file : Tito_Scwrl_5EY2.pdb: