Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYP-ELVHRVESSR----VVAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEA--GVLVNGMGGTPLMEQFVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW 1UN9 Chain:B ((5-340)) ------------FFNQRTHLVSDVIDGAIIASPWNNLARLESDPAIRIVVRRDLNKNNVAVISGGGSGHEPAHVGFIGKGMLTAAVCGDVFASPSVDAVLTAIQAVTGEAGCLLIVKNYTGDRLNFGLAAEKARRLGYNVEMLIVGDDISLPDNKH---PRGIAGTILVHKIAGYFAERGYNLATVLREAQYAASNTFSLGVALSSCHLPQETDAAPRHHPGHAELGMGIHGEPGASVIDTQNSAQVVNLMVDKLL---AALPETGRLAVMINNLGGVSVAEMAIITRELASSPLHSRIDWLIGP-ASLVTALDMKGFSLTAIVLEESIEK-ALLTEVETSNW

General information: TITO was launched using: RESULT: Template: 1UN9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1874 43486 23.20 137.61 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : 23.20 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_1UN9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UN9-query.scw PDB file : Tito_Scwrl_1UN9.pdb: