Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSRYERQEKIGEGTYGVVYKARDTSTAATVALKRIRLDSEEEGVPCTAIREISLLKELRHENIVKLLDVCHSEHRLTIVFEYLDLDLKKYLDRENGNLDAATVQHFMRDLLRGVAFCHQRSVLHRDLKPQNLLISREKELKLGDFGLGRSFAIPVRKFTNEVVTLWYRPPDVLLGSMQYGPPVDVWSVGCIFSEMATGTPLFAGKNDADQLMRIFRFLGTPNNRVWPSMNQYPNSNNMLSQPEFLQNFEPEWSNVLGSVPGYEKLGCAGVDLLEKLLRYEPSERITAADALNHPYFSLQF 5G6V Chain:B ((2-288)) --ETYIKLDKLGEGTYATVYKGKSKLTDNLVALKEIRLEHEEG-APCTAIREVSLLKDLKHANIVTLHDIIHTEKSLTLVFEYLDKDLKQYLDDCGNIINMHNVKLFLFQLLRGLAYCHRQKVLHRDLKPQNLLINERGELKLADFGLARA------------VTLWYRPPDILLGSTDYSTQIDMWGVGCIFYEMATGRPLFPGSTVEEQLHFIFRILGTPTEETWPGILSNEE----FKTYNYPKYRAEALLS------HAPRLDSDGADLLTKLLQFEGRNRISAEDAMKHPFFLSL-

General information: TITO was launched using: RESULT: Template: 5G6V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1335 -153684 -115.12 -558.85 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -115.12 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.463

(partial model without unconserved sides chains): PDB file : Tito_5G6V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G6V-query.scw PDB file : Tito_Scwrl_5G6V.pdb: