Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGYLKVLSPDGRWETRYVEIDDAKLRIWRTKGDKESSAAVVKELDLKCATLREVSEPNTWAVQPEKAEATYFQADGEERKTEWMDTLRHYNSSSSASEKVTLRDFEKKFVLGKGSYGKVFMVVKKDTDKWYAMKEMSAEKMRQ-AEIKAPFAERIILEEIDHPFIVHLHYSFQEQGNLYMILDLLAGGELFTYIEQ-HAPLDEEVVKFYAAEVALALGYLHSRNIIYRDLKPENVVFDRDGHACLTDFGLAKANVHEP--NAVTYCGTNEYLAPELLKG-----VPHGKAVDWWSLGLMMCEMLFNDLPFYDENPMQMQMKILTEDV--AFPPH-IQITEETKDLIRCLLNKNPER--RLQTLEAFKAHKCFSNLDFGLLEARKLKAPITPD---PNPAHNFAKEFTSEVI-VQNE----SPSQAVVTLAGYTYDRDLSEQEKSPSHSPTIAEELRQRRASMKKSTNGSDAASPPVTGENRTSNSSPAGAPTKQAAAGPVKKVEHHIPAKVAPQAARKKLTGNKSFDKPTK 5OTF Chain:A ((72-409)) ---------------------------------------------------------------------------------------------------QLHREDFEIIKVIGRGAFGEVAVVKMKNTERIYAMKILNKWEMLKRAETACFREERDVLVNGDCQWITALHYAFQDENHLYLVMDYYVGGDLLTLLSKFEDKLPEDMARFYIGEMVLAIDSIHQLHYVHRDIKPDNVLLDVNGHIRLADFGSCLKMNDDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHVTDVSEEAKDLIQRLICSRERRLGQNG-IEDFKKHAFFEGLNWENIR--NLEAPYIPDVSSPSDTSNFDV-------EILPPGSHTGFSGLHLPFIGFTFTTES---------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5OTF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1606 -39876 -24.83 -129.47 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -24.83 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_5OTF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5OTF-query.scw PDB file : Tito_Scwrl_5OTF.pdb: