Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence PHFCEVSVSYENAN-KQKITTATKYVVPP----------GSVDIAV--GSQMYAVTVDETCKRTDSI-------SIPGRFKIDTQGKKLEGNPTENYIRSVVLQLNHRGL---RMVSRPAP 1F3U Chain:A ((2-119)) AERGELDLTGAKQNTGVWLVKVPKYLSQQWAKASGRGEVGKLRIAKTQGRTEVSFTLNEDLANIHDIGGKPASVSAPREHPFVLQSVG--GQTLTVFTESSSDKLSLEGIVVQRAECRPA-

General information: TITO was launched using: RESULT: Template: 1F3U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 195 7417 38.04 78.07 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 38.04 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.217

(partial model without unconserved sides chains): PDB file : Tito_1F3U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1F3U-query.scw PDB file : Tito_Scwrl_1F3U.pdb: