Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence DDAHAV----EDTNNNRRSCYYTIFHRAGREWRQIRQTITQITPNCVVNNIRGIPKFWVDDDCTPNYQKSEEVYIRVTAEGAKNYPEPPGFPSGCSLRGRPGSKWKSWGELTPLKQPMRRG 2HGS Chain:A ((202-303)) PNALVLLIAQEKERNIFDQRAIENELLA-RNIHVIRRTFEDISEKGSLDQ-DR--RLFVD------GQEIAVVYFR-DGYMPRQY-SLQNWEARLLLER-------SHAAKCPDIATQLAG

General information: TITO was launched using: RESULT: Template: 2HGS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 387 25345 65.49 258.62 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 65.49 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.151

(partial model without unconserved sides chains): PDB file : Tito_2HGS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HGS-query.scw PDB file : Tito_Scwrl_2HGS.pdb: