Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---LCTTIQKG------QIFRNILTC--MSEFYIKEE---FSYTVCNGKNYASPNRTILCIWEGHIIHVRLDERCNWTRLEGDQTEELESQNVEFKVLQKCVASKYKLDPPHAWDAGHGVKLG-------- 2W4Y Chain:A ((1-122)) ALGDTLTITLGGSGGTAKVLRKINQDGYTSEYYLPETSSSFRAKVRHTKESVKPNQVQ---------YERHNVEFTETVYASGSTPEFVRQAYVVIRHKVGDVSATVSDLGEALSFYLNEALYGKLIGWES

General information: TITO was launched using: RESULT: Template: 2W4Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 339 18589 54.83 185.89 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 54.83 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.232

(partial model without unconserved sides chains): PDB file : Tito_2W4Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W4Y-query.scw PDB file : Tito_Scwrl_2W4Y.pdb: