Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILASVLRSGPGGGLPLRPLLGPALALRARSTSATDTHHVEMARERSKTVTSFYNQSAIDAAAEKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPI--KDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIE----DEKLVRYFLDKTLTSRLGIRMLATHHLALHEDK-----PDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRING------HVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMHGFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHIDGREESFRI 3CRL Chain:B ((18-367)) --------------------------------------------------------KYIEHFSKFSPSPLSMKQFLDFGSSNAC--EKTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCSVSDVVKDAYDMAKLLCDKYYMASPDLEIQEVNATNATQPIHMVYVPSHLYHMLFELFKNAMRATVESHESS-LTLPPIKIMVALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAP-------------------------LAGFGYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDS-----

General information: TITO was launched using: RESULT: Template: 3CRL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1368 -25811 -18.87 -79.91 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -18.87 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.569

(partial model without unconserved sides chains): PDB file : Tito_3CRL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CRL-query.scw PDB file : Tito_Scwrl_3CRL.pdb: