Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MILRHLGI-S------PT-NDLVAKKIFSNPEITCQFIRDMLDLPAKNVTILEGSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV-NQNLEKIHQREGDTH 2ES9 Chain:A ((11-110)) TAIEKALDFIGGMNTSASVPHSMDESTAKGILKYLHD--------LGVPVSPEVVVARGEQE-----GWNPEFTKKVAGWAEKVASGNRILIK----NPEYF---------STYMQEQLKELVLEH----

General information: TITO was launched using: RESULT: Template: 2ES9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 342 15581 45.56 179.09 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 45.56 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.325

(partial model without unconserved sides chains): PDB file : Tito_2ES9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ES9-query.scw PDB file : Tito_Scwrl_2ES9.pdb: