Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFVWYNLFLKKEREVISMRKWTKGFLIFGV-VTTVIGFILLFVGIQSDGIKSLLSMSKEPVYDSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQ-----IHISY--HPSLSAHHDLITNQNDRTLS-----LTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLP-KGRTLKGINISANRGQTTIINASLENATLNTNSYILRIE-GSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAENIQVHGKVELTAKDYLRII------------LDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYKTEKTDVKDQLIARSDDNIDLISTPSRR- 1GVF Chain:A ((2-285)) -----SIIS---TKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSPVILAGTPGTFK----------------HIALEEIYALCSAYSTTYNMPLALHLDHHE--SLDDIRRKVHA-------GVRSAMIDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGS-----------AFLTDPQEAKRFVELT----GVDSLAVAIGTAHG-LYSKTPKIDFQRLAEIREVV---------DVPLVLHGASDVPDEFVRRTIELGVTKVNVATELKIAFAGAVKAWFAENP-----------------QGNDPRYYMRVGMDAMKEVVRNKINVCGSANRIS

General information: TITO was launched using: RESULT: Template: 1GVF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1264 10052 7.95 41.03 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 7.95 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.151

(partial model without unconserved sides chains): PDB file : Tito_1GVF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GVF-query.scw PDB file : Tito_Scwrl_1GVF.pdb: