Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MELVLPNNYVDLEQEEMMYLDGGGVGRNWWNSRGSF----ATVLDVGLAIYSGGATIY--SAYAIKKAISANRGAITRTLRSLIIKHVG--------SAAGHLVNTALNVALTVTGFSLGGAIAYGADWADGSLDGYIFA 5HTL Chain:A ((5-145)) KLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRAHVDIDAVPLLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNQLPDYGFEF--VIAPEKQLVDGFDRYY---

General information: TITO was launched using: RESULT: Template: 5HTL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 401 -23300 -58.10 -192.56 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -58.10 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.62 QMean score : -0.001

(partial model without unconserved sides chains): PDB file : Tito_5HTL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HTL-query.scw PDB file : Tito_Scwrl_5HTL.pdb: