Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIEIKRIQQQPDLAQDIYAVMAAVYPVSPWTLEQIQADLSQDQTWYALAYDGAEVIGFLTVQETLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKE------IFLEVRQSNQRAQAFYKKEKMAVIAERKAYYHDPV----EDAIIMKREIDEG 2CNS Chain:B ((53-142)) --------------------------------------------------------AFAITQVVLDEATLFNIAVDPDFQRRGLGRMLLEHLIDELETRGVVTLWLEVRASNAAAIALYESLGFNEATIRRNYY--PTAQGHEDAIIM-------

General information: TITO was launched using: RESULT: Template: 2CNS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 232 -20050 -86.42 -250.63 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -86.42 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.211

(partial model without unconserved sides chains): PDB file : Tito_2CNS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CNS-query.scw PDB file : Tito_Scwrl_2CNS.pdb: