Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLA-NAPTVLEAYQIVSSIHR-R----NSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQALRNR-----TPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLAILCNYANNLANTPINPELQPYA 2QEU Chain:B ((9-136)) ----------TSQDILKQHAAHYESDMG-GLPEALVQLAEYAPETFDAYSRMRTTMLKSEADGAKLPLKYKHLILVVLDAIRDEPIGIVNHTRAAM-NAGLSVDELIEGILLGIIVYGMPAWGKTGRKAVTFAVEFEKEL-----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2QEU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 445 -7136 -16.03 -60.99 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain B : 0.57 3D Compatibility (PKB) : -16.03 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.370

(partial model without unconserved sides chains): PDB file : Tito_2QEU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QEU-query.scw PDB file : Tito_Scwrl_2QEU.pdb: