Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRQTPSLSFEVFPPTPAVGNDNIISALQDMQELAPHFISVTASNNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALRGDIIPDVEPQKDFRYATDLIELIKERAPHFDIIGACYPEGHPDSPNQISDIQNLKKKVDAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIHLPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDIAGVHLYTMNNADTAKYIHQATHALFNHQSLG 1ZPT Chain:C ((24-286)) ------NVSFEFFPPRTSEMEQTLWNSIDRLSSLKPKFVSVTYGANS-GERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTIARDYWNNGIRHIVALRGDL----------MYASDLVTLLKEVA-DFDISVAAYPEVHPEAKSAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQAKKFADMT-NVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYTLNRAEMSYAI--------------

General information: TITO was launched using: RESULT: Template: 1ZPT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1290 -180006 -139.54 -705.91 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -139.54 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_1ZPT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZPT-query.scw PDB file : Tito_Scwrl_1ZPT.pdb: