Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLNQLDIIVSNVPQVCADLEHILDKKADYADD------GFAQFTIGS----HCLMLSQNHLV-PLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWG-TESLLVQGPAGLVLDFYRMK 4MTS Chain:A ((1-126)) MRILHSMLRVADLEAALEFYTRALDMRLLRRRDYPEGRFTLAFVGYQDERAAAALELTHNWDRDGYTQGDGYGHLAIEVEDAAVTCARARALGYRVTREAGLMQHGRSVIAFLEDPDGYKVELIQK-

General information: TITO was launched using: RESULT: Template: 4MTS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 394 -15727 -39.92 -137.96 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -39.92 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.583

(partial model without unconserved sides chains): PDB file : Tito_4MTS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MTS-query.scw PDB file : Tito_Scwrl_4MTS.pdb: