Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVAAQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFLTHAK----EGYYNGITFHRVIDGFMVQTGD-PKGDGTGGQSIWHDKDKTKDKGTGFKNEITPYLYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLDGKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS 1H0P Chain:A ((21-162)) ----------------------------------------------------------------------------------GRIVIGLFGKTVPKTATNFIELAKKPKGEGY-PGSKFHRVIADFMIQGGDFTRGDGTGGRSIYGEK---------FADENFKLKHYGAGWLSMANAGA-DTNGSQFFITTVKT------------------------PWLDGRHVVFGKILEGMDVVRKIEQTEKLPGDRPKQDVII-------------

General information: TITO was launched using: RESULT: Template: 1H0P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 664 22021 33.16 160.74 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 33.16 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_1H0P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H0P-query.scw PDB file : Tito_Scwrl_1H0P.pdb: