Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIENASYPLTNCGERTAIFKAISEGQREFSELIVYGQ-TEKPISPCGACRQVMAEFFEQDLKVTLVAKDKSTVEMTVGELLPYSFTDLN 2FR5 Chain:C ((24-137)) -----------EAKKSAYCPYSRFPVGAALLTGDGRIFSGCNIENACYPLGVCAERTAIQKAISEGYKDFRAIAISSDLQEEFISPCGACRQVMRE-FGTDWAVYMTKPDGTFVVRTVQELLPASF----

General information: TITO was launched using: RESULT: Template: 2FR5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 554 -26656 -48.12 -235.89 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -48.12 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_2FR5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FR5-query.scw PDB file : Tito_Scwrl_2FR5.pdb: