Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MLYIWMPETNGVWHWSNGENWLQAASLDQLIQDLQIHQGKEAAVFFPSRHAQMLQQTMAKFHYKQLGADGVKYLLEEFVTLPIDHMKVVHHFHADQLTVLAVA-QGMVETWQHSLALLPTK---LVALLPDFLVLPEPQAQQV--------ILCNIGHQLLVRENKWLGNSIDDLGLFLEFQS--AETHYQYSGLTAEQLE-SLEAASSAEQRSEFVYQFQPLDKTKQHPFNVLPKSKGQERTFSNYWKACAAVVLAIIVVQFSYDLLRWVKLKKVADQTAEQAIEQYKYWFGSSSGRVTEQNVRSNFKSRLEVSQQGDTQALSLLSRVGPILMQRQILAQQLNYDASILTMALKTKSADDLQALTQQLNQQGFKAELGNVQADGSGAIGVVKIQ 4D0P Chain:A ((16-386)) NLYFQGGGRMAAAVRQDLAQLMNSSGSHKDLAGKYRQILEKAIQLSGAEQLEALKAFVEAMVNENVSLVISRQLLTDFCTHLPN----LPDSTAKEIYHFTLEKIQPRVISFEEQVASIRQHLASIYEKEEDWRNAAQVLVGIPLETGQKQYNVDYKLETYLKIARLYLEDDDPVQAEAYINRASLLQNESTNEQLQIHYKVCYARVLDYRRKFIEAAQRYNELSY------KTIVH----------ESERLEALKHALHCTILASAGQQRSRMLATLFKDERCQQLAAYGILEKMYL------DRIIRGNQLQEFAAMLMPHQKATTADGSSILDRAVIEHNLLSASKLYNNITFEELGALLEIPAAKAEKIASQMITEG---RMNGFIDQIDG---IVHFE

General information: TITO was launched using: RESULT: Template: 4D0P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1472 1627 1.10 4.67 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 1.10 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.246

(partial model without unconserved sides chains): PDB file : Tito_4D0P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D0P-query.scw PDB file : Tito_Scwrl_4D0P.pdb: