Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTFVDAARSFRTQFDLNFSEKIIVDFFAGGGGASTGLEMGLNRPV---YVAVNHNPKAISMHEANHPHAKHYIQDVFAVDPIDICDGHQVGWFHASPDCTHHSQAA--GGQPRKKEIRDLSWVVLK-FAG--KVKPDVISLENVKQILGWGPLIAKRDKATGRVITLDKININGKKVNRIAEPGERVPRHNQFLVPNPKKKGKTWKHFVRSLEQLGYEVEWQKNIIAADFGAPTKRERLFLIARCDGQPIVWPEKYFSKKPKGNLKKWRSTVECVDFSDLGNSIFDRPQGPLADATLKRIAKGIQKYVIETKEPFFVNSSTPFIGRDFRTSFGHDIREPLATTTAGYGGHSSLISPILVPFITEFANASQQRNWSIDEPLSTICAQVKGGHHGLVTAKLSKDNYKGALRVAAFLINYYGNGDARSITEPMDTITTKDRLVLVTVWIKGEPWAIVDICIRMLKPRELFRAQGVPDSYVIEYGSDGKPLSKKDQVFMVGNSVSPYPMAAIARANNPFITQQIKGAA 3QV2 Chain:A ((8-203)) ------------------QKQVNVIEFFSGIGGLRSSYERS-SININATFIPFDINEIANKIYSKNFKEEV-QVKNLDSISIKQIE-SLNCNTWFMSPPCQPYNNSIMSKHKDINDPRAKSVLHLYRDILPYLINKPKHIFIENVPLFKESL----------------------------------------------------VFKEIYNILIKNQYYIKDII-CSPIDIGIPNSRTRYYVMARLTPFKNEIQ--LH-------QEKESMISNYLDNN------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3QV2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 833 4430 5.32 23.56 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 5.32 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_3QV2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QV2-query.scw PDB file : Tito_Scwrl_3QV2.pdb: