Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRDTVFLKVESRLGYDFSGDIKIGGKYTSLIEHAGLVFISGQIPRVGDTVQICGKVGLDVDLSDAQLAASISTMRALAILKQHYGTLDVIGKVLQMNVFVHSTSSFIQQSEVADGASEILYEILGLDTGRHTRTSVSVYQLPKNASVEINFIFALK 1QD9 Chain:C ((4-124)) ---------------VHTKHAPAAIGPYSQGIIVNNMFYSSGQIPLTPSGEMVNG----DI-KEQ----THQVFSNLKAVLEEAGASFE---TVVKATVFIADMEQFAEVN-------EVYGQYFD-T-HKPARSCVEVARLPKDALVEIEVIALVK

General information: TITO was launched using: RESULT: Template: 1QD9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 591 -66485 -112.49 -549.46 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.68 3D Compatibility (PKB) : -112.49 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.374

(partial model without unconserved sides chains): PDB file : Tito_1QD9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QD9-query.scw PDB file : Tito_Scwrl_1QD9.pdb: